Journal: Frontiers in Oncology
Article Title: Combined Association of Tumoral PD-L1 Expression and Pretreatment Presence of Epstein-Barr Virus DNA With Risk Stratification and Prognosis of Patients With Nasopharyngeal Carcinoma
doi: 10.3389/fonc.2021.791411
Figure Lengend Snippet: Representative immunohistochemistry of (A) negative, (B) weak, (C) moderate, and (D) strong PD-L1 staining in NPC tumor tissues. All the images are 40X magnification, The definition of negative was no staining, weak staining was yellow particles, moderate staining was yellow–brown, and strong staining was brown.
Article Snippet: The sections were stained overnight at 4°C with anti-PD-L1 primary antibody (clone SP142, ZSGB-Bio, Beijing, China), followed by incubation with secondary antibody for 45 min at 37°C and immunoreactions were visualized using the ChemMate EnVision+ detection kit (Peroxidase/Dab, Rabbit/Mouse, K5007, Dako, Glostrup, Denmark).
Techniques: Immunohistochemistry, Staining